Immunohematology Boot Camp: HTLA (21EL-632)

Please note: AABB reserves the right to make updates to this program.

Wednesday, May 5, 2021
2:00 – 3:00 PM Eastern Time
Program Number: 21EL-632

  • Educational Track: Technical/Clinical
  • Topics: Antibody Identification, Immunohematology, Patient Transfusion, Special Patients and Situations, Transfusion Safety
  • Intended Audience: Hospital Blood Banks, Immunohematology Reference Labs (IRL’s), Laboratory Staff, Students (MD, MT, SBB), Technologists
  • Teaching Level: Basic, Intermediate
  • Director/Moderator: Danielle E. Mullins, MSTM MLS(ASCP)CM SBBCM,  Supervisor, Immunohematology Reference Laboratory, Versiti Illinois, Aurora, IL

    • Maddie Knier, MLS(ASCP)CM SBBCM, Senior Scientist, Immunohematology Reference Laboratory, Versiti Wisconsin, Milwaukee, WI
    • Suzanne M. Davisson, BS SBB(ASCP)CM, Senior Scientist, Immunohematology Reference Laboratory, Versiti Indiana, Indianapolis, IN
  • Program Description

    This program is intended as an introduction and/or refresher on “high titer low avidity” (HTLA) reactivity that often present a challenge to identify and work around. Within the lecture portion of the program, learners will receive background on the blood group antigens associated with these tricky reactivity patterns. Learners will also be presented with methods and techniques used in the laboratory to identify these antibodies. Case studies will conclude the session to provide examples of how these antibodies may present within a patient case.

    Learning Objectives

    After participating in this educational activity, participants should be able to:

    • Describe the concept of “high titer low avidity antibodies” (HTLA) and associate appropriate blood group antigens with this type of reactivity. (Chido/Rodgers blood group, Knops blood group, Cost collection, JMH, Sda, Bgs)
    • Discuss the serologic characteristics, clinical significance, and therapeutic implications of the antibodies directed toward the antigens listed above.
    • Discuss the laboratory methods used to differentiate these serologically difficult antibodies including titration, urine and plasma neutralization, and enzyme sensitivity.
    • Identify reactivity patterns associated with these blood group antigens within the context of a case study.


    Registration includes access to both the live and on-demand version of this eCast.

    AABB understands the value of learning together as a group but with COVID-19, bringing everyone together to share the experience in person may not be an option for your team. Given this, all participants in a group will be provided the ability to register as a single viewer (AABB will provide a promocode to registered group viewing coordinators to share with their team in advance of the live program). If you are a facility interested in participating in this eCast, simply complete the Group Viewing registration form and AABB will provide detailed instructions to share with your team. For single viewers, the process has not changed – simply click on the Register button below to register.

    Please note: registration for the live eCast will close 1 hour prior to the eCast start time. If you register after this time you will receive access to the on-demand eCast when it is available.

    Single Viewer Register
    Group Viewing Register

    Continuing Education Credit

    AABB estimates both the live and on-demand version of this eCast each to be eligible for 1 continuing education credits/contact hours for Physicians, California Nurse, California Lab Personnel, Florida Lab Personnel and General Participation credit. The final number of credit(s) and credit types awarded for this program (live or on-demand) will be determined by the program duration. For more information on each credit type please visit our Continuing Education Credits webpage.

    Disclosures for the planners of this event can be found here. Disclosures for the program faculty are provided at the beginning of the program.

    Speaker Biographies

    Maddie Knier is a senior scientist in the Immunohematology Reference Lab at Versiti Wisconsin. She has previously worked for Duke University Transfusion Services and Children’s Hospital of Wisconsin Transfusion Services before transferring to her current position within the IRL in 2013. Maddie obtained her SBB certification in 2014 and has worked on many projects within the lab. Her interests include ABO sequencing and teaching MLS and SBB students in complex antibody identifications.

    Suzanne Davisson has been an Immunohematology Reference Laboratory technologist for 16 years and has a special interest in teaching and training students and new technologists. She earned a Bachelor of Science in Molecular Genetics from The Ohio State University and her Specialist in Blood Banking certification while working at The American Red Cross in Columbus, Ohio. She has been with Versiti Indiana for the past 11 years in Indianapolis, where she lives with her husband, son and new goldendoodle puppy, Dakota.